Vimentin
Expected Turnaround Time
2 days
Stated turn-around-times (TATs) are for clinical use only and subject to change based on biopharma protocol requirements. Final TATs will be specified in the biopharma study contract.
Stated turn-around-times (TATs) are for clinical use only and subject to change based on biopharma protocol requirements. Final TATs will be specified in the biopharma study contract.
Clinical Information
Vimentin is an intermediate filament protein that provides structural support to cells and is primarily expressed in mesenchymal cells, including fibroblasts, endothelial cells, and smooth muscle cells. In pathology, vimentin is widely used to identify tumors of mesenchymal origin, such as sarcomas, and to differentiate these from epithelial and other types of malignancies.
Requisition Forms
Specimen Requirements
Specimen Requirements
- Fresh Bone Marrow Aspirate: 4 mL (0.5 mL), EDTA or NaHep
- Bone Marrow Aspirate Smear (pre-prepared): 4-6 slides (min 1 slide), Methanol fixed preferred (Air dried accepted)
- Fresh Peripheral Blood: 4 mL (0.5 mL), EDTA or NaHep
- Peripheral Blood Smear (pre-prepared): 4-6 slides (min 1 slide), Methanol fixed preferred (Air dried accepted)
- Bone Marrow Core Biopsy – wet tissue: 1 cm; fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin (NBF) or B-Plus fixative
- Bone Marrow Aspirate Clot – wet tissue: 5 mm; fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin (NBF) or B-Plus fixative
- Formalin-fixed, Paraffin-Embedded (FFPE) Cores and Clots (preprepared): 4-6 slides; 3- 5 μM on adhesion glass (min 1 slide per stain), Formalin Fixed
- Fresh Tissue Biopsies: Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin (NBF) for 6-72 hours (48-72 hours PREFERRED)
- Fresh Tissue Resections: Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin (NBF) for 6-72 hours (48-72 hours PREFERRED)
- Formalin-fixed Paraffin Embedded Tissue (FFPE blocks): FFPE Block, Formalin Fixed
- Unstained slides: 2 slides (3-5 μM) per stain (min 1 slide per stain), Formalin Fixed
- Stained slides: 1 slide per stain, Formalin Fixed
Specimen Stability
- Fresh Tissue Tissues: ≤ 72 Hours
- Formalin-fixed Paraffin Embedded Tissue (FFPE blocks): Indefinite
- Unstained slides: Indefinite
Storage Requirements
15°C to 25°C
Shipping Conditions
Ambient
Shipping Recommendations
- Fresh Tissues: AMBIENT
- Formalin-fixed Paraffin Embedded Tissue (FFPE blocks) and unstained slides: AMBIENT, Use a refrigerated (NOT FROZEN) gel pack in the shipment to protect from extreme temperatures. Separate gel pack from specimen.
- Specimens may be transferred to 70% alcohol for transportation purposes after fixation in 10% NBF or B plus.
Specimen Rejection Criteria
Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected.
Test Details
Test Method
Immunohistochemistry
Methodology Category
IHC
Regulatory Status
IVD, CE-marked
Special Considerations
- Fresh Tissues: Specimens may be fixed in formalin for 24-72 hours by collection sites, then transferred to 70% ethanol and stored at 2-8°C for longer term storage if needed. Samples must be tested 3 months after placing in ethanol.
- Unstained slides: Positively charged glass slides required to prevent background staining and prevent tissue loss. Slides should be left unbaked.