Thyroid Transcription Factor-1
Expected Turnaround Time
2 days
Stated turn-around-times (TATs) are for clinical use only and subject to change based on biopharma protocol requirements. Final TATs will be specified in the biopharma study contract.
Stated turn-around-times (TATs) are for clinical use only and subject to change based on biopharma protocol requirements. Final TATs will be specified in the biopharma study contract.
Clinical Information
TTF-1 is a nuclear protein crucial for the development of the thyroid, lung, and brain. TTF-1 is used to differentiate between lung adenocarcinomas and other malignancies, as its expression is strongly positive in non-small cell lung cancer, particularly adenocarcinoma, and in thyroid tumors. It is also valuable in identifying metastatic tumors to the lung, as TTF-1 positivity helps distinguish primary lung cancers from other types of carcinomas.
Requisition Forms
Specimen Requirements
Specimen Requirements
- Fresh Tissues: Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin (NBF) for 6-72 hours (48-72 hours PREFERRED)
- Formalin-fixed Paraffin Embedded Tissue (FFPE blocks): FFPE Block, Formalin Fixed
- Unstained slides: 2 slides (3-5 μM) per stain (min 1 slide per stain), Formalin Fixed
Specimen Stability
- Fresh Tissue Tissues: ≤ 72 Hours
- Formalin-fixed Paraffin Embedded Tissue (FFPE blocks): Indefinite
- Unstained slides: Indefinite
Storage Requirements
15°C to 25°C
Shipping Conditions
Ambient
Shipping Recommendations
- Fresh Tissues: AMBIENT
- Formalin-fixed Paraffin Embedded Tissue (FFPE blocks) and unstained slides: AMBIENT, Use a refrigerated (NOT FROZEN) gel pack in the shipment to protect from extreme temperatures. Separate gel pack from specimen.
- Specimens may be transferred to 70% alcohol for transportation purposes after fixation in 10% NBF or B plus.
Specimen Rejection Criteria
Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected.
Test Details
Synonyms
NK2 homeobox 1 (NKX2-1), Homeobox Protein NK-2 Homolog A, Homeobox Protein Nkx-2.1, TTF-1, Thyroid nuclear factor 1, Thyroid-specific enhancer-binding protein (T/EBP)
Test Method
Immunohistochemistry
Methodology Category
IHC
Regulatory Status
IVD, CE-marked
Special Considerations
- Fresh Tissues: Specimens may be fixed in formalin for 24-72 hours by collection sites, then transferred to 70% ethanol and stored at 2-8°C for longer term storage if needed. Samples must be tested 3 months after placing in ethanol.
- Unstained slides: Positively charged glass slides required to prevent background staining and prevent tissue loss. Slides should be left unbaked.