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FIP1L1/PDGFRA 4q12 Rearrangement

Test ID: F 4Q12 CPT Code: 88374 (if automated read), 88377 (if manual read)
Expected Turnaround Time
5 days
Stated turn-around-times (TATs) are for clinical use only and subject to change based on biopharma protocol requirements. Final TATs will be specified in the biopharma study contract.
Clinical Information
FISH test on hematologic specimens for detection of FIP1L1/CHIC2/PDGFRA (4q12) deletion and gene fusion. This probe is used for detecting a microdeletion involving the CHIC2 locus that leads to fusion of the FIP1L1 and PDGFRA genes. This abnormality is most frequently observed in eosinophilia and myeloproliferative neoplasms. Often ordered as part of the F EOSINOPHILA Panel.
Requisition Forms
Oncology Pathology Requisition

Specimen Requirements

Specimen Requirements
  • Whole Blood: 5 mL, EDTA preferred (NaHep accepted)
  • Bone Marrow Aspirate: 3 mL, EDTA preferred (NaHep accepted)
Specimen Stability

72 hours

Storage Requirements
  • Whole Blood: 2°C to 25°C
  • Bone Marrow Aspirate: 2°C to 25°C
  • Fixed Cytogenetically Prepared Cells: -28°C to -15°C
Shipping Conditions
Ambient, Refrigerated
Shipping Recommendations

AMBIENT, Use a refrigerated (NOT FROZEN) gel pack in the shipment to protect from extreme temperatures. Separate gel pack from specimen. Do not freeze.

Specimen Rejection Criteria

Clotted specimen; Specimen exposed to extreme temperature; Anticoagulant toxic to cells; Insufficient number of cells; Improper fixative.

Test Details

Synonyms

4q12 rearrangement, CHIC2/F1LP1L1/PDGFRα, platelet-derived growth factor receptor-α, platelet-derived growth factor receptor alpha, FIP1, RHE, PDGFR2, RHEPDGFRA, FIP1L1-PDGFRA fusion

Keywords
Pathology, Fluorescence in-situ Hybridization, Leukemia, Oncology, Oncology FISH Probes, Hypereosinophilia / Mast Cell
Test Method
Fluorescence in situ hybridization (FISH)
Methodology Category
FISH
Regulatory Status
ASR
Special Considerations

All attempts will be made to process and report samples received > 72 hours post-collection.  Bone marrow is the sample of choice in most heme malignancies.

Associations

Hypereosinophilia, Mast cell