Herpes Simplex Virus Type 1 & 2
Expected Turnaround Time
1-3 days
Stated turn-around-times (TATs) are for clinical use only and subject to change based on biopharma protocol requirements. Final TATs will be specified in the biopharma study contract.
Stated turn-around-times (TATs) are for clinical use only and subject to change based on biopharma protocol requirements. Final TATs will be specified in the biopharma study contract.
Clinical Information
Qualitative real-time PCR assay for the detection of herpes simplex virus types 1 and 2 (HSV-1 and HSV-2). HSV-1 is primarily linked to oral lesions and HSV-2 to genital lesions, though both can affect either region.
Requisition Forms
Specimen Requirements
Specimen Requirements
- Cervical cells: 2.0 mL (min. 1.0 mL), ThinPrep® PAP solution or SurePath™ Test Pack solution
- Swab from lesion: viral transport media
- Fresh or frozen tissue: 5 mm3
- CSF: 2.0 mL (min. 1.0 mL), sterile container, DO NOT DILUTE
- Whole blood: 2.0 mL minimum, EDTA preferred
Specimen Stability
- Cervical cells: 18-25°C, ambient
- Swab from lesion site: 2-8°C, stable 96 hours
- Fresh tissue: 2-8°C, stable 96 hours
- Frozen tissue: ≤ -15°C, freeze immediately
- CSF: 2-8°C, stable 24 hours
- Whole blood: 2-8°C, stable 30 days
Shipping Recommendations
- Cervical cells: AMBIENT
- Swab (lesion site): REFRIGERATED, Protect from extreme temperature with ice pack. Separate ice pack from specimen.
- Fresh tissue: REFRIGERATED, Protect from extreme temperature with ice pack. Separate ice pack from specimen.
- Frozen tissue: FROZEN, Ship samples on dry ice. Separate dry ice from specimens.
- CSF: REFRIGERATED, Protect from extreme temperature with ice pack. Separate ice pack from specimen.
- Whole blood: REFRIGERATED, Protect from extreme temperature with ice pack. Separate ice pack from specimen.
Specimen Rejection Criteria
Specimens beyond stability; recommended temperatures exceeded; improper transport media; improper anticoagulant
Test Details
Synonyms
HSV-1, HSV-2
Keywords
Pathology, Molecular Diagnostics, Women's Health, STI
Test Method
Real-time polymerase chain reaction (RT-PCR)
Methodology Category
Molecular
Regulatory Status
RUO
Special Considerations
- Fresh tissue: submit in transport medium such as RPMI, Hanks, or equivalent.
- Frozen tissue: freeze within 4 hours of collection
Associations
Detection of Herpes simplex virus (HSV) by polymerase chain reaction (PCR) has proven to be the most specific, rapid and sensitive means to diagnose anogenital and central nervous system infections.
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