ROS1 6q22.1 Rearrangement, paraffin
Expected Turnaround Time
5 days
Stated turn-around-times (TATs) are for clinical use only and subject to change based on biopharma protocol requirements. Final TATs will be specified in the biopharma study contract.
Stated turn-around-times (TATs) are for clinical use only and subject to change based on biopharma protocol requirements. Final TATs will be specified in the biopharma study contract.
Clinical Information
FISH testing on paraffin-embedded cells for detection of ROS1 (6q22.1) gene rearrangement. ROS1 rearrangements occur in a subset of non-small cell lung cancers (NSCLCs) and, less frequently, in glioma. Often ordered with FP ALK.
Specimen Requirements
Specimen Requirements
- FFPE Tissue Block
- Unstained FFPE tissue: 5 slides preferred (3 slides minimum) cut at 3-5 μM and mounted on positively charged slides.
Specimen Stability
Stable indefinitely
Storage Requirements
15°C to 25°C
Shipping Conditions
Ambient
Shipping Recommendations
AMBIENT, Use a refrigerated (NOT FROZEN) gel pack in the shipment to protect from extreme temperatures. Separate gel pack from specimen. Do not freeze.
Specimen Rejection Criteria
Improper fixative; Tissue not fixed; Insufficient tissue; Specimen exposed to extreme temperature
Test Details
Synonyms
6q22.1 rearrangement; Receptor Tyrosine Kinase (RTK); Non-small cell lung cancer
Keywords
Pathology, Fluorescence in-situ Hybridization, Molecular Diagnostics, Oncology, Oncology FISH Probes, Mutation Analysis, Bladder Cancer, Lung Cancer
Test Method
Fluorescence in situ hybridization (FISH)
Methodology Category
FISH
Regulatory Status
ASR
Special Considerations
Tissues should be fixed in 10% neutral buffered formalin (NBF). Tissues preserved in B+ fixative or decalcified are usually not suitable for FISH. Assay requires 3 unstained slides minimum (two for each probe) and one H&E stained slide. If unstained slides are sent, cut FFPE tissue sections at 3-5 μm thickness, mounted on positively charged slides.
Associations
Lung cancer