BCL6 3q27 Rearrangement, paraffin
Expected Turnaround Time
5 days
Stated turn-around-times (TATs) are for clinical use only and subject to change based on biopharma protocol requirements. Final TATs will be specified in the biopharma study contract.
Stated turn-around-times (TATs) are for clinical use only and subject to change based on biopharma protocol requirements. Final TATs will be specified in the biopharma study contract.
Clinical Information
FISH testing on paraffin-embedded cells for detection of BCL6 (3q27) gene rearrangement. BCL6 rearrangements occur in diffuse large B-cell lymphoma, follicular center cell lymphoma, and marginal zone B-cell lymphoma. Often ordered as part of the FP BURKITT Panel test.
Specimen Requirements
Specimen Requirements
- FFPE Tissue Block
- Unstained FFPE tissue: 5 slides preferred (3 slides minimum) cut at 3-5 μM and mounted on positively charged slides.
Specimen Stability
Stable indefinitely
Storage Requirements
15°C to 25°C
Shipping Conditions
Ambient
Shipping Recommendations
AMBIENT, Use a refrigerated (NOT FROZEN) gel pack in the shipment to protect from extreme temperatures. Separate gel pack from specimen. Do not freeze.
Specimen Rejection Criteria
Improper fixative; Tissue not fixed; Insufficient tissue; Specimen exposed to extreme temperature
Test Details
Synonyms
BCL6 gene; 3q27 rearrangement
Keywords
Fluorescence in-situ Hybridization, Oncology, Oncology FISH Probes, Lymphoma
Test Method
Fluorescence in situ hybridization (FISH)
Methodology Category
FISH
Regulatory Status
ASR
Special Considerations
Tissues should be fixed in 10% neutral buffered formalin (NBF). Tissues preserved in B+ fixative or decalcified are usually not suitable for FISH. Assay requires 3 unstained slides minimum (two for each probe) and one H&E stained slide. If unstained slides are sent, cut FFPE tissue sections at 3-5 μm thickness, mounted on positively charged slides.
Associations
B-cell lymphoma, Non-Hodgkin lymphoma (NHL), Diffuse large B cell, Follicular lymphoma