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Burkitt/"Double Hit" Large B-cell Lymphoma Panel, paraffin

Test ID: FP BURKITT CPT Code: 88377 x4

Expected Turnaround Time
5 days
Stated turn-around-times (TATs) are for clinical use only and subject to change based on biopharma protocol requirements. Final TATs will be specified in the biopharma study contract.

Clinical Information
FISH Panel testing on paraffin-embedded cells for the most common abnormalities observed in Burkitt and high-grade B-cell lymphoma. Includes: c-MYC (8q24) rearrangement, BCL6 (3q27) rearrangement, IGH::MYC fusion, and IGH::BCL2 fusion.

Requisition Forms

Specimen Requirements


Specimen Requirements
  • FFPE Tissue Block
  • Unstained FFPE tissue: 7 slides preferred (5 slides minimum) cut at 3-5 μM and mounted on positively charged slides. 

Specimen Stability

Stable indefinitely


Storage Requirements

15°C to 25°C


Shipping Conditions
Ambient

Shipping Recommendations

AMBIENT, Use a refrigerated (NOT FROZEN) gel pack in the shipment to protect from extreme temperatures. Separate gel pack from specimen. Do not freeze.


Specimen Rejection Criteria

Improper fixative; Tissue not fixed; Insufficient tissue; Specimen exposed to extreme temperature


Test Details


Synonyms

C-MYC 8q24, IGH::MYC, BCL2 t(14;18), BCL6 3q27


Keywords
Fluorescence in-situ Hybridization, Oncology, Lymphoma, Oncology FISH Panels

Test Method
Fluorescence in situ hybridization (FISH)

Methodology Category
FISH

Regulatory Status
ASR

Special Considerations

Tissues should be fixed in 10% neutral buffered formalin (NBF). Tissues preserved in B+ fixative or decalcified are usually not suitable for FISH. Assay requires 5 unstained slides minimum (one for each probe) and one H&E stained slide. If unstained slides are sent, cut FFPE tissue sections at 3-5 μm thickness, mounted on positively charged slides. 


Associations

"double hit" B-cell lymphoma