EGFR 7p12 Amplification*, paraffin

Test ID: FP EGFR* CPT Code: 88377

Connect with an Expert

Expected Turnaround Time

9 days
Stated turn-around-times (TATs) are for clinical use only and subject to change based on biopharma protocol requirements. Final TATs will be specified in the biopharma study contract.

Clinical Information

FISH testing on paraffin-embedded cells for detection of EGFR (7p11.2) gene amplification. Amplification of EGFR is a recurrent abnormality in a variety of cancers, including non-small cell lung cancer, metastatic colorectal cancer, and glioblastoma. *Testing performed by affiliate laboratory.

Specimen Requirements

FFPE Tissue Block
Unstained FFPE tissue: 5 slides preferred (3 slides minimum) cut at 3-5 μM and mounted on positively charged slides.

Specimen Stability

Stable indefinitely

Storage Requirements

15°C to 25°C

Shipping Conditions

Ambient

Shipping Recommendations

AMBIENT, Use a refrigerated (NOT FROZEN) gel pack in the shipment to protect from extreme temperatures. Separate gel pack from specimen. Do not freeze.

Specimen Rejection Criteria

Improper fixative; Tissue not fixed; Insufficient tissue; Specimen exposed to extreme temperature

Test Details

Synonyms

EGFR (HER1/erb-B1), CEP 7

Keywords

Fluorescence in-situ Hybridization, Oncology, Oncology FISH Probes, Breast Cancer, Gastrointestinal Cancer, Lung Cancer, Neural

Test Method

Fluorescence in situ hybridization (FISH)

Methodology Category

FISH

Special Considerations

Tissues should be fixed in 10% neutral buffered formalin (NBF). Tissues preserved in B+ fixative or decalcified are usually not suitable for FISH. Assay requires 3 unstained slides minimum (two for each probe) and one H&E stained slide. If unstained slides are sent, cut FFPE tissue sections at 3-5 μm thickness, mounted on positively charged slides.

Associations

Brain, Breast, lung and colon cancer

Get Expert Guidance on EGFR 7p12 Amplification*, paraffin

Our laboratory specialists can help you understand test requirements, methodology, and interpretation.

Connect with an Expert Today