PBMC Isolation

Test ID: G PBMC CPT Code: N/A

Biopharma: This test is available for use in clinical trials or research purposes only.

Expected Turnaround Time

1 day
Stated turn-around-times (TATs) are for clinical use only and subject to change based on biopharma protocol requirements. Final TATs will be specified in the biopharma study contract.

Clinical Information

Peripheral blood mononuclear cell (PBMC) processing involves the isolation of mononuclear cells, including lymphocytes and monocytes, from whole blood through density gradient centrifugation. This process is used to assess immune responses, biomarker identification, and drug efficacy.

Specimen Requirements

Whole blood collected in NaHep, EDTA, or LiHep (dependent on protocol).

Specimen Stability

Best results achieved if processing begins within 4 hours of blood collection.

Storage Requirements

Store at room temperature prior to PBMC isolation. Isolated PBMCs stored in liquid nitrogen long term.

Shipping Conditions

Ambient

Shipping Recommendations

AMBIENT, Use a refrigerated (NOT FROZEN) gel pack in the shipment to protect from extreme temperatures. Separate gel pack from specimen.

Specimen Rejection Criteria

Clotted specimens; whole blood collected in the wrong anti-coagulant; hemolyzed or clotted specimens

Test Details

Synonyms

Peripheral Blood Mononuclear Cell

Test Method

Cell Processing

Methodology Category

Molecular

Regulatory Status

RUO