Gordon & Sweets Special Stain (Reticulum), technical only
Biopharma: This test is available for use in clinical trials or research purposes only.
Expected Turnaround Time
2 days
Stated turn-around-times (TATs) are for clinical use only and subject to change based on biopharma protocol requirements. Final TATs will be specified in the biopharma study contract.
Stated turn-around-times (TATs) are for clinical use only and subject to change based on biopharma protocol requirements. Final TATs will be specified in the biopharma study contract.
Clinical Information
Gordon and Sweet's reticulin stain is a silver impregnation technique used to visualize reticular fibers, which are a type of connective tissue fiber. This method results in black-stained fibers against a pale background. It is particularly useful in liver histopathology for assessing the architecture of the liver and identifying conditions such as cirrhosis. Technical component only. Pathologist read not included.
Specimen Requirements
Specimen Requirements
- Fresh Tissue: Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin (NBF) for 6-72 hours (48-72 hours PREFERRED)
- Formalin-fixed Paraffin Embedded Tissue (FFPE blocks): FFPE Block, Formalin Fixed
- Unstained slides: 2 slides (3-5 μM) per stain (min 1 slide per stain), Formalin Fixed
Specimen Stability
- Formalin-fixed, Paraffin-Embedded (FFPE) tissues: Indefinite
- Fresh Tissue tissues: ≤ 72 Hours
- Unstained slides: Indefinite
Storage Requirements
15°C to 25°C
Shipping Conditions
Ambient
Shipping Recommendations
- Fresh Tissue: AMBIENT
- Formalin-fixed Paraffin Embedded Tissue (FFPE blocks) and unstained slides: AMBIENT, Use a refrigerated (NOT FROZEN) gel pack in the shipment to protect from extreme temperatures. Separate gel pack from specimen.
- Specimens may be transferred to 70% alcohol for transportation purposes after fixation in 10% NBF or B plus.
Specimen Rejection Criteria
Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected.
Test Details
Test Method
Anatomic Pathology
Methodology Category
Anatomic Pathology
Regulatory Status
IVD, CE-marked
Special Considerations
- Fresh Tissue: Specimens may be fixed in formalin for 6-72 hours by collection sites, then transferred to 70% ethanol and stored at 2-8°C for longer term storage if needed. This is NOT preferred. Samples should be tested 3 months after placing in ethanol.
- Unstained slides: Positively charged glass slides required to prevent background staining and prevent tissue loss. Slides should be left unbaked.