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AML1/ETO t(8;21)

Test ID: F AML ETO CPT Code: 88374 (if automated read) 88377 (if manual read)

Expected Turnaround Time
5 days
Stated turn-around-times (TATs) are for clinical use only and subject to change based on biopharma protocol requirements. Final TATs will be specified in the biopharma study contract.

Clinical Information
FISH test on hematologic specimens for detection of RUNX1::RUNX1T1 gene fusion. This fusion, associated with t(8;21), is commonly observed in acute myeloid leukemia.

Requisition Forms

Specimen Requirements


Specimen Requirements
  • Whole Blood: 5 mL, EDTA preferred (NaHep accepted)
  • Bone Marrow Aspirate: 3 mL, EDTA preferred (NaHep accepted)

Specimen Stability

72 hours


Storage Requirements
  • Whole Blood: 2°C to 25°C
  • Bone Marrow Aspirate: 2°C to 25°C
  • Fixed Cytogenetically Prepared Cells: -28°C to -15°C

Shipping Conditions
Ambient, Refrigerated

Shipping Recommendations

AMBIENT, Use a refrigerated (NOT FROZEN) gel pack in the shipment to protect from extreme temperatures. Separate gel pack from specimen. Do not freeze.


Specimen Rejection Criteria

Clotted specimen; Specimen exposed to extreme temperature; Anticoagulant toxic to cells; Insufficient number of cells


Test Details


Synonyms

AML1/ETO t(8;21)(q22;q22), RUNX1T1/RUNX1


Keywords
Fluorescence in-situ Hybridization, Leukemia, Oncology, Oncology FISH Probes, Acute Myeloid Leukemia (AML)

Test Method
Fluorescence in situ hybridization (FISH)

Methodology Category
FISH

Regulatory Status
ASR

Special Considerations

All attempts will be made to process and report samples received > 72 hours post-collection.  Bone marrow is the sample of choice in most heme malignancies.


Associations

Acute myelogenous leukemia, AML(M2)