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BCL6 3q27 Rearrangement

Test ID: F BCL6 CPT Code: 88374 (if automated read), 88377 (if manual read)

Expected Turnaround Time
5 days
Stated turn-around-times (TATs) are for clinical use only and subject to change based on biopharma protocol requirements. Final TATs will be specified in the biopharma study contract.

Clinical Information
FISH test on hematologic specimens for detection of BCL6 (3q27) gene rearrangement. BCL6 rearrangements occur in diffuse large B-cell lymphoma, follicular lymphoma, and marginal zone B-cell Lymphoma. Often ordered as part of the F BURKITT Panel.

Requisition Forms

Specimen Requirements


Specimen Requirements
  • Whole Blood: 5 mL, EDTA preferred (NaHep accepted)
  • Bone Marrow Aspirate: 3 mL, EDTA preferred (NaHep accepted)

Specimen Stability

72 hours


Storage Requirements
  • Whole Blood: 2°C to 25°C
  • Bone Marrow Aspirate: 2°C to 25°C
  • Fixed Cytogenetically Prepared Cells: -28°C to -15°C

Shipping Conditions
Ambient, Refrigerated

Shipping Recommendations

AMBIENT, Use a refrigerated (NOT FROZEN) gel pack in the shipment to protect from extreme temperatures. Separate gel pack from specimen. Do not freeze.


Specimen Rejection Criteria

Clotted specimen; Specimen exposed to extreme temperature; Anticoagulant toxic to cells; Insufficient number of cells

 


Test Details


Synonyms

BCL6 gene; 3q27 rearrangement, Bcl-6


Keywords
Fluorescence in-situ Hybridization, Oncology, Oncology FISH Probes, Lymphoma

Test Method
Fluorescence in situ hybridization (FISH)

Methodology Category
FISH

Regulatory Status
ASR

Special Considerations

All attempts will be made to process and report samples received > 72 hours post-collection.  Bone marrow is the sample of choice in most heme malignancies.


Associations

B-cell lymphoma, Non-Hodgkin lymphoma (NHL), Diffuse large B cell, Follicular lymphoma