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beta-glucuronidase

Test ID: I B-GLUC CPT Code: N/A
Expected Turnaround Time
2 days
Stated turn-around-times (TATs) are for clinical use only and subject to change based on biopharma protocol requirements. Final TATs will be specified in the biopharma study contract.
Clinical Information
ß-glucuronidase is localized to the lysosome where it plays a critical role in the degradation of complex carbohydrates. Literature, and pathology resources demonstrate strong staining in normal liver and colon tissue, intermediate staining in normal tonsil and testis, and intermediate-to-weak staining in normal gallbladder. Overexpression may be observed across tumor types, especially B cell malignancies. All staining is shown to be localized to lysosomes in a punctate expression pattern.
Requisition Forms
Immunohistochemistry Requisition

Specimen Requirements

Specimen Requirements
  • Fresh Tissues: Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin (NBF) for 6-72 hours (48-72 hours PREFERRED)
  • Formalin-fixed Paraffin Embedded Tissue (FFPE blocks): FFPE Block, Formalin Fixed
  • Unstained slides: 2 slides (3-5 μM) per stain (min 1 slide per stain), Formalin Fixed
Specimen Stability
  • Fresh Tissue Tissues: ≤ 72 Hours
  • Formalin-fixed Paraffin Embedded Tissue (FFPE blocks): Indefinite
  • Unstained slides: Indefinite
  • Stained slides: Indefinite
Storage Requirements

15°C to 25°C

Shipping Conditions
Ambient
Shipping Recommendations
  • Fresh Tissues: AMBIENT
  • Formalin-fixed Paraffin Embedded Tissue (FFPE blocks) and unstained slides: AMBIENT, Use a refrigerated (NOT FROZEN) gel pack in the shipment to protect from extreme temperatures. Separate gel pack from specimen.
  • Specimens may be transferred to 70% alcohol for transportation purposes after fixation in 10% NBF or B plus.
Specimen Rejection Criteria

Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected. 

Test Details

Synonyms

b-glu; b-gluc; ßGLU; GUSB; Glucuronidase Beta; Beta-G1; Beta-D-Glucuronidase; Glucuronidase, Beta 2; BG

Test Method
Immunohistochemistry
Methodology Category
IHC
Regulatory Status
RUO
Special Considerations
  • Fresh Tissues: Specimens may be fixed in formalin for 24-72 hours by collection sites, then transferred to 70% ethanol and stored at 2-8°C for longer term storage if needed. This is NOT preferred. Samples must be tested 3 months after placing in ethanol.
  • Unstained slides: Positively charged glass slides required to prevent background staining and prevent tissue loss. Slides should be left unbaked.