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Periodic Acid-Schiff Stain (Fungus)

Test ID: S PAS-F CPT Code: 88312
Expected Turnaround Time
2 days
Stated turn-around-times (TATs) are for clinical use only and subject to change based on biopharma protocol requirements. Final TATs will be specified in the biopharma study contract.
Clinical Information
The PAS-fungus special stain is specifically designed to detect fungal organisms by highlighting fungal cell walls. PAS-F is a valuable diagnostic tool in pathology for diagnosing fungal infections, especially those caused by Candida, Aspergillus, and Histoplasma species.

Specimen Requirements

Specimen Requirements
  • Fresh Tissue: Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin (NBF) for 6-72 hours (48-72 hours PREFERRED)
  • Formalin-fixed Paraffin Embedded Tissue (FFPE blocks): FFPE Block, Formalin Fixed
  • Unstained slides: 2 slides (3-5 μM) per stain (min 1 slide per stain), Formalin Fixed
Specimen Stability
  • Formalin-fixed, Paraffin-Embedded (FFPE) tissues: Indefinite
  • Fresh Tissue tissues: ≤ 72 Hours
  • Unstained slides: Indefinite
Storage Requirements

15°C to 25°C

Shipping Conditions
Ambient
Shipping Recommendations
  • Fresh Tissue: AMBIENT
  • Formalin-fixed Paraffin Embedded Tissue (FFPE blocks) and unstained slides: AMBIENT, Use a refrigerated (NOT FROZEN) gel pack in the shipment to protect from extreme temperatures. Separate gel pack from specimen.
  • Specimens may be transferred to 70% alcohol for transportation purposes after fixation in 10% NBF or B plus.
Specimen Rejection Criteria

Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected. 

Test Details

Synonyms

Periodic Acid-Schiff Fungus; PAS-F

Test Method
Anatomic Pathology
Methodology Category
Anatomic Pathology
Regulatory Status
IVD, CE-marked
Special Considerations
  • Fresh Tissue: Specimens may be fixed in formalin for 6-72 hours by collection sites, then transferred to 70% ethanol and stored at 2-8°C for longer term storage if needed. This is NOT preferred. Samples should be tested 3 months after placing in ethanol.
  • Unstained slides: Positively charged glass slides required to prevent background staining and prevent tissue loss. Slides should be left unbaked.