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BRG1

Test ID: I BRG1 CPT Code: N/A
Expected Turnaround Time
2 days
Stated turn-around-times (TATs) are for clinical use only and subject to change based on biopharma protocol requirements. Final TATs will be specified in the biopharma study contract.
Clinical Information
BRG1 (SWI / SNF related, matrix associated, actin dependent regulator of chromatin, subfamily A, member 4) is a protein involved in chromatin remodeling, which is important for regulating the binding of transcription factors to DNA. Loss of protein expression as well as overexpression are associated with several distinct neoplasms.
Requisition Forms
Immunohistochemistry Requisition

Specimen Requirements

Specimen Requirements
  • Fresh Tissues: Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin (NBF) for 6-72 hours (48-72 hours PREFERRED)
  • Formalin-fixed Paraffin Embedded Tissue (FFPE blocks): FFPE Block, Formalin Fixed
  • Unstained slides: 2 slides (3-5 μM) per stain (min 1 slide per stain), Formalin Fixed
Specimen Stability
  • Fresh Tissue Tissues: ≤ 72 Hours
  • Formalin-fixed Paraffin Embedded Tissue (FFPE blocks): Indefinite
  • Unstained slides: Indefinite
  • Stained slides: Indefinite
Storage Requirements

15°C to 25°C

Shipping Conditions
Ambient
Shipping Recommendations
  • Fresh Tissues: AMBIENT
  • Formalin-fixed Paraffin Embedded Tissue (FFPE blocks) and unstained slides: AMBIENT, Use a refrigerated (NOT FROZEN) gel pack in the shipment to protect from extreme temperatures. Separate gel pack from specimen.
  • Specimens may be transferred to 70% alcohol for transportation purposes after fixation in 10% NBF or B plus.
Specimen Rejection Criteria

Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected. 

Test Details

Synonyms

SMARCA4; hSNF2ß; Actin-like protein 6B; 53 kDa BRG1-associated factor B; Actin-related protein Baf53b; ArpNalpha; BRG1-associated factor 53B; BAF53B; SWI/SNF Related, Matrix Associated; Actin Dependent Regulator Of Chromatin, Subfamily A Member; Mitotic Growth and Transcription Activator; ATP-Dependent Helicase 
SNF2-BETA; HSNF2b; BAF190; SNF2LB; SNF2L4; SNF2; SWI2; Global Transcription Activator Homologous Sequence; Sucrose Nonfermenting-Like 4; Transcription Activator BRG1; BRG1-Associated Factor 190A; Protein Brahma Homolog 1; Homeotic Gene Regulator; Brahma Protein-Like 1; Nuclear Protein GRB1; Protein BRG-1; SNF2-Like 4; FLJ39786; SWI/SNF-Related Matrix-Associated Actin-Dependent Regulator of Chromatin Subfamily A Member 4; SNF2-Beta 4; OTSC12; MRD16; RTPS2; SNF2B; CSS4 

Test Method
Immunohistochemistry
Methodology Category
IHC
Regulatory Status
RUO
Special Considerations
  • Fresh Tissues: Specimens may be fixed in formalin for 24-72 hours by collection sites, then transferred to 70% ethanol and stored at 2-8°C for longer term storage if needed. This is NOT preferred. Samples must be tested 3 months after placing in ethanol.
  • Unstained slides: Positively charged glass slides required to prevent background staining and prevent tissue loss. Slides should be left unbaked.