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PRDM16 1p36 Rearrangement

Test ID: F PRDM16 CPT Code: 88374 (if automated read), 88377 (if manual read)

Expected Turnaround Time
5 days
Stated turn-around-times (TATs) are for clinical use only and subject to change based on biopharma protocol requirements. Final TATs will be specified in the biopharma study contract.

Clinical Information
FISH test on hematologic specimens for detection of PRDM16 (1p36.32) gene rearrangement. PRDM16 rearrangement is a rare but recurrent finding in cases of acute myeloid leukemia and myelodysplastic syndrome.

Requisition Forms

Specimen Requirements


Specimen Requirements
  • Whole Blood: 5 mL, EDTA preferred (NaHep accepted)
  • Bone Marrow Aspirate: 3 mL, EDTA preferred (NaHep accepted)

Specimen Stability

72 hours


Storage Requirements
  • Whole Blood: 2°C to 25°C
  • Bone Marrow Aspirate: 2°C to 25°C
  • Fixed Cytogenetically Prepared Cells: -28°C to -15°C

Shipping Conditions
Ambient, Refrigerated

Shipping Recommendations

AMBIENT, Use a refrigerated (NOT FROZEN) gel pack in the shipment to protect from extreme temperatures. Separate gel pack from specimen. Do not freeze.


Specimen Rejection Criteria

Clotted specimen; Specimen exposed to extreme temperature; Anticoagulant toxic to cells; Insufficient number of cells


Test Details


Synonyms

KIAA1675, MEL1, PFM13, t(1;3)(p36;q21)


Keywords
Fluorescence in-situ Hybridization, Leukemia, Oncology, Oncology FISH Probes, acute myeloid leukemia (AML), myelodysplastic syndrome (MDS)

Test Method
Fluorescence in situ hybridization (FISH)

Methodology Category
FISH

Regulatory Status
RUO

Special Considerations

All attempts will be made to process and report samples received > 72 hours post-collection.  Bone marrow is the sample of choice in most heme malignancies.


Associations

acute myeloid leukemia (AML), myelodysplastic syndrome (MDS)