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Trisomy 12

Test ID: F T12 CPT Code: 88374 (if automated read), 88377 (if manual read)

Expected Turnaround Time
5 days
Stated turn-around-times (TATs) are for clinical use only and subject to change based on biopharma protocol requirements. Final TATs will be specified in the biopharma study contract.

Clinical Information
FISH test on hematologic specimens for detection of Trisomy 12. Trisomy 12 is a common recurring chromosomal defect in chronic lymphocytic leukemia (CLL). It has been reported in 15–30% of cases and is correlated with atypical morphology.

Specimen Requirements


Specimen Requirements
  • Whole Blood: 5 mL, EDTA preferred (NaHep accepted)
  • Bone Marrow Aspirate: 3 mL, EDTA preferred (NaHep accepted)

Specimen Stability

72 hours


Storage Requirements
  • Whole Blood: 2°C to 25°C
  • Bone Marrow Aspirate: 2°C to 25°C
  • Fixed Cytogenetically Prepared Cells: -28°C to -15°C

Shipping Conditions
Ambient, Refrigerated

Shipping Recommendations

AMBIENT, Use a refrigerated (NOT FROZEN) gel pack in the shipment to protect from extreme temperatures. Separate gel pack from specimen. Do not freeze.


Specimen Rejection Criteria

Clotted specimen; Specimen exposed to extreme temperature; Anticoagulant toxic to cells; Insufficient number of cells


Test Details


Synonyms

+12, CEP 12


Keywords
Fluorescence in-situ Hybridization, Leukemia, Oncology, Oncology FISH Probes, Chronic Lymphocytic Leukemia (CLL)

Test Method
Fluorescence in situ hybridization (FISH)

Methodology Category
FISH

Regulatory Status
ASR

Special Considerations

All attempts will be made to process and report samples received > 72 hours post-collection.  Bone marrow is the sample of choice in most heme malignancies.


Associations

Chronic Lymphocytic Leukemia (CLL)