TM4SF1 ISH
Biopharma: This test is available for use in clinical trials or research purposes only.
Expected Turnaround Time
3 days
Stated turn-around-times (TATs) are for clinical use only and subject to change based on biopharma protocol requirements. Final TATs will be specified in the biopharma study contract.
Stated turn-around-times (TATs) are for clinical use only and subject to change based on biopharma protocol requirements. Final TATs will be specified in the biopharma study contract.
Clinical Information
TM4SF1 is a transmembrane protein involved in cell adhesion and signaling, with a role in cancer cell migration and metastasis. In pathology, TM4SF1 is used to help identify aggressive tumors, particularly in breast cancer, where its overexpression correlates with poor prognosis and increased metastatic potential. This assay detects TM4SF1 mRNA using RNAScope ISH technology.
Requisition Forms
Specimen Requirements
Specimen Requirements
- Fresh Tissues: Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin (NBF) for 6-36 hours (24 hours PREFERRED)
- Formalin-fixed Paraffin Embedded Tissue (FFPE blocks): FFPE Block, Formalin Fixed
- Unstained slides: 5 slides (3-5 μM) per stain (min 3 slides), Formalin Fixed
Specimen Stability
- Fresh Tissue Tissues: ≤ 36 Hours
- Formalin-fixed Paraffin Embedded Tissue (FFPE blocks): Indefinite
- Unstained slides: 2 weeks
Storage Requirements
15°C to 25°C
Shipping Conditions
Ambient
Shipping Recommendations
- Fresh Tissues: AMBIENT
- Formalin-fixed Paraffin Embedded Tissue (FFPE blocks) and unstained slides: AMBIENT, Use a refrigerated (NOT FROZEN) gel pack in the shipment to protect from extreme temperatures. Separate gel pack from specimen.
- Specimens may be transferred to 70% alcohol for transportation purposes after fixation in 10% NBF or B plus.
Specimen Rejection Criteria
Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected.
Test Details
Synonyms
Transmembrane 4 L Six Family Member 1, Transmembrane 4 L6 family member 1, Membrane component chromosome 3 surface marker 1, Tumor-associated antigen L6
Test Method
In Situ Hybridization (ISH)
Methodology Category
IHC
Regulatory Status
RUO
Special Considerations
- Fresh Tissues: Specimens should be fixed for 16-36 hours to prevent RNA degradation.
- Unstained slides: Positively charged glass slides required to prevent background staining and prevent tissue loss. Slides should be left unbaked.
- FFPE blocks or fresh tissue is preferred.