Acute Myeloid Leukemia (AML) Secondary Panel

Test ID: F AML Secondary CPT Code: 88374 x5 (if automated read), 88377 (if manual read)

Expected Turnaround Time

5 days
Stated turn-around-times (TATs) are for clinical use only and subject to change based on biopharma protocol requirements. Final TATs will be specified in the biopharma study contract.

Clinical Information

FISH panel test on hematologic specimens for abnormalities observed in acute myeloid leukemia (AML) that occur with less frequency than the abnormalities included on the AML Frontline Panel. Includes: MECOM rearrangement, NUP98 rearrangement, DEK::NUP214 fusion, BCR::ABL1 fusion and TP53 (17p13) deletion. Frequently ordered in conjunction with AML Frontline Panel or as a reflex test if AML Frontline Panel is negative.

Requisition Forms

Oncology Pathology Requisition

Specimen Requirements

Whole Blood: 5 mL, EDTA preferred (NaHep accepted)
Bone Marrow Aspirate: 3 mL, EDTA preferred (NaHep accepted)

Specimen Stability

72 hours

Storage Requirements

Whole Blood: 2°C to 25°C
Bone Marrow Aspirate: 2°C to 25°C
Fixed Cytogenetically Prepared Cells: -28°C to -15°C

Shipping Conditions

Ambient, Refrigerated

Shipping Recommendations

AMBIENT, Use a refrigerated (NOT FROZEN) gel pack in the shipment to protect from extreme temperatures. Separate gel pack from specimen. Do not freeze.

Specimen Rejection Criteria

Clotted specimen; Specimen exposed to extreme temperature; Anticoagulant toxic to cells; Insufficient number of cells

Test Details

Keywords

Fluorescence in-situ Hybridization, Leukemia, Oncology, Oncology FISH Probes, Acute Myeloid Leukemia (AML)

Test Method

Fluorescence in situ hybridization (FISH)

Methodology Category

FISH

Regulatory Status

ASR

Special Considerations

All attempts will be made to process and report samples received > 72 hours post-collection. Bone marrow is the sample of choice in most heme malignancies.

Associations

Acute myelogenous leukemia