B7-H3

Test ID: I B7-H3 CPT Code: N/A

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Biopharma: This test is available for use in clinical trials or research purposes only.

Expected Turnaround Time

2 days
Stated turn-around-times (TATs) are for clinical use only and subject to change based on biopharma protocol requirements. Final TATs will be specified in the biopharma study contract.

Clinical Information

B7-H3 is a novel member of the B7 immune protein family. B7-H3 activates or inhibits tumor immune responses by binding to receptors on the surface of immune cells. Apart from participating in tumor immune activities, it has regulatory effects on non-immunological functions, such as tumor migration and invasion, angiogenesis, glycometabolism, and drug resistance. Thus, it has important biological functions in regulating the progression of malignant tumors. Zhao, S., Zhang, H., & Shang, G. (2025).

Specimen Requirements

Fresh Tissues: Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin (NBF) for 6-72 hours (48-72 hours PREFERRED)
Formalin-fixed Paraffin Embedded Tissue (FFPE blocks): FFPE Block, Formalin Fixed
Unstained slides: 2 slides (3-5 μM) per stain (min 1 slide per stain), Formalin Fixed

Specimen Stability

Fresh Tissue Tissues: ≤ 72 Hours
Formalin-fixed Paraffin Embedded Tissue (FFPE blocks): Indefinite
Unstained slides: Indefinite
Stained slides: Indefinite

Storage Requirements

15°C to 25°C

Shipping Conditions

Ambient

Shipping Recommendations

Fresh Tissues: AMBIENT
Formalin-fixed Paraffin Embedded Tissue (FFPE blocks) and unstained slides: AMBIENT, Use a refrigerated (NOT FROZEN) gel pack in the shipment to protect from extreme temperatures. Separate gel pack from specimen.
Specimens may be transferred to 70% alcohol for transportation purposes after fixation in 10% NBF or B plus.

Specimen Rejection Criteria

Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected.

Test Details

Synonyms

CD276

Test Method

Immunohistochemistry

Methodology Category

IHC

Regulatory Status

RUO

Special Considerations

Fresh Tissues: Specimens may be fixed in formalin for 24-72 hours by collection sites, then transferred to 70% ethanol and stored at 2-8°C for longer term storage if needed. This is NOT preferred. Samples must be tested 3 months after placing in ethanol.

Unstained slides: Positively charged glass slides required to prevent background staining and prevent tissue loss. Slides should be left unbaked.

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